The present study was conducted to assess the endocrine status of serum testosterone, estradiol and thyroid hormones in good fertility breeding buffalo bulls and their male calves. Fifteen buffalo bulls were categorized into good fertility bulls on the basis of semen evaluation and reproductive history. Thirty one male calves were selected from these fi fteen buffalo bulls. Average testosterone concentration was higher (p<0.05) in adult buffalo bulls than male calves (2.93 ± 0.44 ng/ml versus 1.20 ± 0.35 ng/ ml). Similarly estradiol concentration in adult buffalo bull was (p<0.05) higher (51.66 ± 2.54 pg/ml) as compared to male calves (15.352 ± 2.47 pg/ml). Serum TSH was found higher (p<0.05) in adult buffalo bulls(18.83 ± 4.15 µIU/ml) than their male calves (7.08 ± 2.48 µIU/ml) but serum concentrations of T3 and T4 were similar in adult buffalo bull and their male calves (5.84 ± 0.60 ng/ml and 4.96 ± 0.52 µg/dl versus 3.73 ± 0.38 ng/ml and 3.34 ± 0.29 µg/dl, respectively)